Senior Research Assistant II/ Lab Manager

Company: Joslin Diabetes Center
Location: Boston
Posted on: May 29, 2023

Job Description:

The main focus of the Beta Cell Aging Lab is to understand the contribution of cellular aging to diabetes in animal and human models of disease to find new therapeutic targets that can reverse dysfunction and early cellular death. The Senior Research Assistant II/Lab Manager will assist in the analysis of insulin secretion, mechanisms of aging and diverse mouse models. The Senior Research Assistant II/Lab Manager will work closely with the Principal Investigator (Cristina Aguayo-Mazzucato) in experimental design and development, oversight of day-to-day laboratory operations, and coordination of experiments with lab members. Responsibilities:Work with PI in developing experimental design, evaluation, and troubleshooting for all members of the lab. Conduct all the regulated lab protocols revision/renewal, including animal study, COMS and human study; and act as the resource person in the lab. Develop new laboratory protocol(s) and participate in various lab projects. Contribute to data generation, analysis, presentation(s), and manuscripts. In absence of PI, perform managerial duties. Working closely with the Office of Sponsored Research and Finance departments, provide oversight of lab financials and assist with budget planning. Assist in the recruitment/new hire process. This includes coordinating search with Human Resources, interviewing candidates, and providing feedback/recommendation(s) to PI. Train and supervise the Research Assistants. Provide feedback to PI during annual performance review process. Arrange and coordinate all lab shipments, including those for animal and samples. Maintain critical reagent inventory and sample storage. Train Research Assistants/Students/Fellows/Technicians on mouse colony maintenance - including: numbering, tailing, breeding, weighing, monitoring food intake, responses to medication, injecting via the intra-peritoneal and intravenous routes, and performing oral gavages. Perform and train members of the lab on RNA, DNA, and protein isolation, Western, Northern and, PCR, tissue culture, immunohistochemistry and gene chips. Maintain and perform an islet perifusion system to measure insulin secretion from cell lines, animal and human islets. Supervise and review lab data records, quality assurance, and ensure the lab is adhering to proper practices. Ensure that basic operational needs of the lab are met, including: ordering supplies and general lab maintenance. Especially, the status of equipment/instruments, ensuring they adhere to their calibration/maintenance schedules. Allocate equitable research lab space for incoming post-doctoral fellows and research personnel, and perform overall supervision of lab equipment by research personnel.Perform other duties as assigned.Qualifications:Bachelor's degree in biochemistry, biology, molecular biology or related field.Master's degree in biochemistry, biology, molecular biology or related field.Minimum 5 years' of experience with biochemical, molecular and cellular studies as well as experience working with small animals (mice and rats).Minimum 2 years' supervisory experience.Advanced knowledge of Microsoft Office SuiteExperience and comfort in handling mice and rats, and maintenance of animal coloniesStrong interpersonal skills and excellent verbal and written communication skillMust be able to work independentlyStrong knowledge of Microsoft Office Suite and ability to use databases and spreads Joslin is an Equal Opportunity Employer of Minority/Female/Individuals with Disabilities/Protected Veterans. Joslin values diversity and inclusion, and we encourage diversity applicants.Joslin Diabetes Center is an Equal Opportunity Employer and it is the policy of Joslin to prohibit discrimination of any type and to afford equal employment opportunities to employees, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, genetic information, marital/parental status, disability status, protected veteran status or any other status protected by law.PDN-99274006-c4ec-4ebf-b727-030732eb9e91

Keywords: Joslin Diabetes Center, Boston , Senior Research Assistant II/ Lab Manager, Executive , Boston, Massachusetts