Research Tech - Senior
Company: Dana-Farber Cancer Institute
Posted on: June 13, 2021
We are seeking a highly motivated candidate to fill a full-time
research technician position in Dr. David Weinstock's laboratory at
the Dana-Farber Cancer Institute. We are a translational leukemia
and lymphoma research laboratory that uses a variety of
biochemical, molecular, and cell biology approaches to identify new
therapeutic targets, develop preclinical models of disease, and
test novel therapeutics both in vitro and in vivo. The research
technician would work on a project studying mechanisms of response
and resistance to JAK2 kinase inhibition in B-cell acute
lymphoblastic leukemia and myeloproliferative neoplasms. The
successful candidate will be a team player capable of handling
multiple tasks and adaptable to a fast-paced research
Located in Boston and the surrounding communities, Dana-Farber
Cancer Institute brings together world renowned clinicians,
innovative researchers and dedicated professionals, allies in the
common mission of conquering cancer, HIV/AIDS and related diseases.
Combining extremely talented people with the best technologies in a
genuinely positive environment, we provide compassionate and
comprehensive care to patients of all ages; we conduct research
that advances treatment; we educate tomorrow's
physician/researchers; we reach out to underserved members of our
community; and we work with amazing partners, including other
Harvard Medical School-affiliated hospitals.
The senior technician will be responsible for conducting
experiments to advance the research progress in the lab. As part of
this work, they will be responsible for the following:
- Maintenance of cell lines in culture, testing novel
therapeutics using in vitro proliferation assays, and generation of
drug-resistant cell lines.
- Basic handling of DNA and RNA including sample isolation, PCR,
and preparation of samples for sequencing.
- Protein isolation from cells and primary murine samples,
Western blotting, and immunoprecipitation experiments.
- CRISPR guide RNA design and molecular cloning.
- Transfection and retroviral / lentiviral transduction of murine
and human cells for overexpression experiments or CRISPR-Cas9
- Multi-color flow cytometry.
- Breeding mice and testing novel therapeutics in mouse models of
leukemia. Previous experience working with mice is required.
- Analyzing genomic datasets commensurate with previous
experience. Previous experience in bioinformatics would be an
asset, but is not required.
- Preparation of reagents and media used in lab experiments.
- Maintaining detailed records of their laboratory work, results,
and data analyses.
- Sharing responsibilities for operation and maintenance of
standard lab equipment.
The technician should be able to work independently, but also be
open and receptive to suggestions and feedback from the team /
project leader. Attention to detail in completing tasks and a
strong work ethic is essential. This includes willingness to
critically read and keep up to date on the latest scientific
literature, logically troubleshoot problems, offer both
intellectual and technical input during collaborative meetings, and
assist in the preparation of presentations, grants, and
manuscripts. The technician should have flexibility in their
schedule to work on some evenings and weekends as needed to meet
project goals. We seek candidates who have at least 2 years of
- Bachelor's or Master's degree in Biology, Biochemistry,
Chemistry, or a related field is required.
- At least 2 years minimum of previous molecular biology
laboratory research experience and familiarity with the majority of
the techniques listed above required.
- Excellent communication skills and ability to synthesize
research findings for presentation in both oral and written
- At least 1-2 years minimum of previous experience and
familiarity with handling of mice is required.
Dana-Farber Cancer Institute is an equal opportunity employer
and affirms the right of every qualified applicant to receive
consideration for employment without regard to race, color,
religion, sex, gender identity or expression, national origin,
sexual orientation, genetic information, disability, age, ancestry,
military service, protected veteran status, or other groups as
protected by law.
Keywords: Dana-Farber Cancer Institute, Boston , Research Tech - Senior, Other , Boston, Massachusetts
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