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Under supervision and direction in a line to the Clinical Lab
Supervisor and the Technical Director of the Immunopathology,
incumbent is responsible for performing all assays for autoantibody
detection in patients with suspected or actual Wegener's
granulomatosis or other related vasculities. The diagnostic
information is used by clinicians for treatment and patient care
Assists in the day-to-day operations and overseeing of the ANCA
laboratory of the Immunopathology Unit. Trains new technologists on
proper procedures and techniques, evaluates new technology for
clinical application and assists in updating procedure manuals for
laboratory inspections and accreditation such JCAHO.
Functions as principal technical specialist for the ANCA
laboratory, provides instruction in special techniques, methodology
and the reading and interpreting of test results to students,
residents; provides all of the above except the reading and
interpreting of test results to the pathologists.
Responsible for performing indirect immunofluorescence and ELISA
(immunoassay) for detection of circulating autoantibodies such as
ANCA (anti-neutrophil cytoplasmic antibodies) which are associated
with life threatening diseases and syndromes that involve the lung
and kidney. Responsible for quality control of reagents including
Utilizes phlebotomy techniques for obtaining normal blood donors
for the isolation of neutrophils. Prepares cytosmears of the
isolated neutrophils to be used for indirect
Isolates proteinase-3 (p-29), one of the antigens in the
immunoassay (ELISA), by using affinity column chromatography
Assessions samples, logs data, files reports and orders
Coats microtiter plates with antigens, blocks plates and
performs the indirect or direct ELISA assay. Washes plates,
develops in enzyme substrate and reads optical densities using
ELISA plate reader. Aliquots sera and freezes them after assay is
Additional duties include operation and maintenance of all
laboratory equipment as well as development and implementation of
new and improved techniques and protocols to ensure high quality
results. Additionally, the technologist actively participates in
teaching and training of pathology residents and fellows. Instructs
pathology residents in the reading and interpretation of test
The technologist also maintains and updates the laboratory
manual in accordance with the JCAHO regulations and implements
hospital safety standards with the help of the Technical
Workflow and workload of diagnostic specimens may require
working Friday evenings
Maintains quality control logs on a variety of equipment.
BS or BA. in Medical Technology, Clinical Laboratory Science or
Chemical, Physical, or Biological Science and with MT ASCP
Certification and 3-5 years experience .
Familiarity with immunology, computers, and the metric system.
Knowledge of chemistry; familiarity with the concepts of molarity,
normality, molecular weight, formula weight of chemicals.
Familiarity with how to make dilutions from stock solutions.
Knowledge of good laboratory technique. Must be able to do
mathematical calculations, and read/write English. Must know proper
handling procedure of chemicals.
Effective interpersonal and communication skills, attention to
detail. Must be organized and capable of following protocols and
have ability to devise or adapt techniques if needed to achieve
desired goals. Supervisory skills are needed to instruct
technologist or residents. Ability to recognize novel or emergency
situations and respond appropriately. Good hand and eye
coordination and manual dexterity. Ability to work well with