Research Scientist, NGS Assay Development

Company: Takeda Pharmaceutical
Location: Boston
Posted on: September 30, 2024

Job Description:

By clicking the "Apply" button, I understand that my employment application process with Takeda will commence and that the information I provide in my application will be processed in line with Takeda's and . I further attest that all information I submit in my employment application is true to the best of my knowledge.Job DescriptionAbout the role:At Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as a Research Scientist where you will use your knowledge in NGS assay development to help accelerate the discovery and development of biologics therapeutics. You will also establish workflows utilizing next-generation sequencing technologies to advance antibody discovery efforts from in vitro (yeast and phage), in vivo (single/bulk B cells) or de novo (AI based) platforms with the goal to more deeply interrogate repertoires, expand our knowledge of functional and sequence diversity and accelerate lead discovery. As part of the Biologics Informatics and Automation team, you will report to the Head of the team and work with computational scientists, establish data analysis pipelines for NGS data, investigating the use of NGS in other areas in Global Biologics to accelerate workflows and to enhance pipeline support and accelerate programs to IND.How you will contribute:

• Develop and optimize NGS protocols for library generation, sequencing using Illumina MiSeq/NextSeq platforms, and library QC and analysis.
• Collaborate with cross-functional teams to integrate NGS data with antibody discovery pipelines.
• Establish workflows using NGS in other areas in Global Biologics including cell line generation, plasmid preparation and CAR-T design characterization.
• Generate high quality NGS library (e.g. RNASeq, CITE-Seq) by rigorous optimization and QC process.
• Perform DNA library fragment purification using Blue Pippin and analyze the size using Tape Station 4200.
• Library quantification using qubit and qPCR.
• Continuously improve NGS workflows with integration of automation and informatics.
• Deeply understand and troubleshoot NGS experiments to ensure assay robustness and generation of high-quality data.
• Meet established timelines, maintain accurate records of work, and present data at team, departmental and company-wide meeting.Minimum Requirements/Qualifications:
  • PhD degree in Molecular Biology, Biochemistry or relevant scientific field (or equivalent) with 2+ years relevant experience, or MS with 8+ years relevant experience, or BS with 10+ years relevant experience.
  • Minimum 3 years of industry experience of hands-on NGS library generation and operating Illumina sequencing platform is required.
  • A deep understanding of molecular biology with a focus on RNA isolation, cDNA synthesis, PCR amplification, SPRI bead based clean up and fragment analysis knowledge is a must.
  • Demonstrated ability to design NGS primers and strategy to utilize NGS sequencing tools currently available in the organization.
  • Experience with single cell technologies (10X, BD Rhapsody) is a plus.More about us:At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs. Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work.Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.#LI-SGM#LI-OnsiteTakeda Compensation and Benefits SummaryWe understand compensation is an important factor as you consider the next step in your career. We are committed to equitable pay for all employees, and we strive to be more transparent with our pay practices. -For Location:Boston, MAU.S. Base Salary Range:$108,500.00 - $170,500.00The estimated salary range reflects an anticipated range for this position. The actual base salary offered may depend on a variety of factors, including the qualifications of the individual applicant for the position, years of relevant experience, specific and unique skills, level of education attained, certifications or other professional licenses held, and the location in which the applicant lives and/or from which they will be performing the job.---The actual base salary offered will be in accordance with state or local minimum wage requirements for the job location. -U.S. based employees may be eligible for short-term and/ or long-term incentives. U.S. based employees may be eligible to participate in medical, dental, vision insurance, a 401(k) plan and company match, short-term and long-term disability coverage, basic life insurance, a tuition reimbursement program, paid volunteer time off, company holidays, and well-being benefits, among others. U.S. based employees are also eligible to receive, per calendar year, up to 80 hours of sick time, and new hires are eligible to accrue up to 120 hours of paid vacation. -EEO StatementTakeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law.LocationsBoston, MAWorker TypeEmployeeWorker Sub-TypeRegularTime TypeFull time

Keywords: Takeda Pharmaceutical, Boston , Research Scientist, NGS Assay Development, Other , Boston, Massachusetts